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In Situ

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Hybridization (ISH) Data

Table of Contents

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Viewing Gene Expression Data

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The following special operators can be used to build queries:

Operator

Example Search Type

Example Query

AND

Gene

dopamine AND receptor

OR

Gene

ABAT OR AChE

NOT

Gene

"dopamine receptor" NOT DRD3

  • AND, OR and NOT must be upper case.
  • The AND function is implicit, so spaces between words that are not separated by an operator will be treated like AND.
  • OR has higher operator precedence than AND.
  • Parentheses can be used to group criteria, but nested parentheses are not supported at this time.

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Searches will return a list of experiments based on the input search criteria. Each row includes the following information.

Column

Description

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Add the experiment to your selections by clicking the checkbox

Fold Change

(Differential Search only) A numeric value representing the fold change between expression of the target structure(s) and the Contrast structure(s)

Experiment

Click the experiment number to view experimental detail page

Gene Symbol

Click Gene Symbol to return gene metadata

Gene Name

Full gene name

Probe Name

Unique identifier for the probe used in this experiment

Orientation

Orientation of the probe: sense or antisense

Plane

The plane of sectioning for the experiment: sagittal or coronal

Expression Summary

Thumbnail view of a histogram showing the expression energy across 12 major brain structures

Select the experiments to view in greater detail by clicking on the checkboxes next to your experiments of interest. Experiments will be saved in your cache until you select the "Clear Selections" button at the bottom of the page. After selecting one or more experiments, click the "View Selections" button.

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Use the toolbar to take actions on the image that currently has focus. Toolbar controls include:

Control

Function

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Move the slider bar to the left to intensify the contrast or color

Image Modified

Select ISH, Nissl or Expression image type

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Display all thumbnail images in a single 'contact sheet'

Image Modified

Open the selected image in the image viewer tool

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Synchronize all other Zap viewers on the page that support synchronization to the currently selected image

Image Modified

Open a new browser window to review metadata for the image series

Image Modified

Close the Zap viewer

Keyboard Commands

Use the keyboard to navigate through the image series and synchronize the viewers on the page. Keyboard commands include:

Key

Function

F

Advance to the next image in the series

D

Go back to the previous image in the series

R

Advance to the last image in the series

E

Go back to the first image in the series

S

Synchronize all Zap viewers on the page that support synchronization to the currently selected image

A

Zoom in

Z

Zoom out

+

Zoom in. Please note that some keyboards may require the [Shift] key be held down while pressing the [+] key

-

Zoom out

You can also use the arrow keys to pan the current image.

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