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From the interactive Transgenic Mouse Lines and Cortical Imaging Locations panels on the landing page, you can explore the transgenic mouse line, cortical area and cortical depth of the experiments conducted in this study. Hovering your mouse over the transgenic lines, cortical area or cortical depth will highlight the parameters captured in the experiments of this study. Clicking on one of the interactive links will return a #Cell Cell Search with those specific criteria.

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Each experiment is displayed by a row in the results list below the Filters menu. All of the columns (minus the two common mouse lines) are displayed by default. Columns can be hidden by deselecting them from the drop-down menu. The population thumbnails are segregated by visual stimulus as indicated by the stimulus color key. Overall cell population responses of the neurons in the imaging field of view to a specific visual stimuli are displayed as population thumbnails and can be explored in more detail from the #Experiment Experiment Detail Page.

 

Name

Description

 

Experiment ID

Clicking on this link will open the #Experiment Experiment Detail Page

 

Brain Area

Brain Area imaged (VISal, VISp, VISl, VISpm)

 

Cre Driver

Cre Driver line used (Rbp4-Cre_KL100, Cux2-CreERT2, Emx1-IRES-Cre, Scnn1a-Tg3-Cre, Rorb-IRES2-Cre, Nr5a1-Cre)

 

Imaging Depth

Imaging depth in microns (175, 250, 275, 335, 350, 375)

 

Cells

Clicking on this link will open a #Cell Cell Search page with cells from this experiment

 

Experiment

Shows a projection image of cells from this experiment - clicking will open this #Experiment Experiment Detail Page

a & f

Orientation Selectivity

Cumulative histogram plot of orientation selectivity of responsive cells during the static and drifting gratings stimuli

b

Preferred Spatial Frequency

Histogram plotting the preferred spatial frequency of responsive cells during the static gratings stimulus

c

Preferred Orientation

Semi-pie chart showing the preferred orientation of responsive cells during the static gratings stimulus

d & i

Time to Peak

Temporal dynamics of the mean response of responsive cells to the static grating and natural scenes stimuli

e

Direction Selectivity

Cumulative histogram plot of direction selectivity of responsive cells during the drifting gratings stimulus

g

Preferred Direction

Pie chart showing the preferred direction of responsive cells during the drifting gratings stimulus

h

Preferred Temporal Frequency

Histogram plotting the preferred temporal frequency of responsive cells during the drifting gratings stimulus

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Metadata for the experiment is listed at the top of the page including transgenic lines, targeted functional visual areas, imaging depth as well as a link to the #Cell Cell List, which identifies each responding cell in the two-photon calcium imaging sessions. Summary images taken from each of the imaging sessions are included under the metadata.

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Below the summary images and metadata are large versions of each of the population thumbnails shown on the #Experiment Experiment Overview page, with a description of what the thumbnail represents.

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This page lists all the relevant cells from the selected experiment and shows the cellular responses at a deeper level. Each row represents a single cell and illustrates the cell response through a series of thumbnails that represent a summary of the cellular response to a visual stimuli. Clicking on a row will take you to the #Cell Cell Detail Page.

More information on the development of each of the thumbnails and it's corresponding visual stimulus can be found from the "Visual Stimuli" buttons on the Overview page.

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  1. Filters: Show, hide or clear filters by clicking on these buttons
  2. Sort: Sort by parameter and choose either ascending or descending sort
  3. Add/Remove Columns: Increase or decrease the number of columns shown in your cell search results
  4. Current Filters: Filters will show in the box once applied
  5. Filter Parameters: Clicking a radio box will determine which visual stimulus or metadata parameters you can filter on
  6. Filter Criteria: Parameters are visual stimulus and metadata dependent. Cell metadata parameters are Brain area, Imaging depth and Cre-driver. You can also limit the data results to cells with data in all columns from this search. Visual stimulus parameters are as follows: p-value (all visual stimuli), Orientation Selectivity (static or drifting gratings), Preferred Orientation (static or drifting gratings), Preferred direction (drifting grating), Preferred phase (static gratings), Preferred spatial frequency (static gratings), Preferred temporal frequency (drifting grating), Time to peak (static grating or natural scene), Preferred image (natural image),
  7. Row Number: The number of cells included in the filter criteria
    More information can be found on these thumbnails and metrics from the #Cell Cell Detail Page

Cell Detail Page

Information at the level of the individual cell is available from the this page. Similar to the #Experiment Experiment Detail Page the top of the page provides metadata from the experiment from which this cell was imaged.

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