Projection Dataset

Searching

This dataset allows you to browse an image database of axonal projections labeled by viral (rAAV) tracers and visualized using serial two-photon tomography. To search this dataset, browse and select from a list of primary injections sites (approximately 27 sites for the November 2011 release). Selecting an injection site will return a list of experiments.

This list outlines the experiment ID, Structure Name, Mouse Strain, Sex, and Age. Clicking on an experiment ID will launch a contact sheet viewer. Clicking on the checkbox next to the experiment will allow you to select one or more experiments to view in more detail by clicking "View Selections". Experiments will continue to be selected until you click the "Clear Selections" button.

Contact Sheet Viewer

The contact sheet viewer shows serial sections of the selected experiment. Background fluorescence in the red channel illustrates basic anatomy and structures of the brain, and the injection site and projections are shown in the green channel.

Double-clicking on individual images on the contact sheet will launch a High Resolution Image Viewer.

High Resolution Image Viewer

Thumbnail images at the bottom of the high resolution image viewer can be selected to display individual sections in the main viewer. The viral tracer is visualized in the green channel, while background auto-fluorescence, seen in the red channel, is useful to provide anatomical context. No information was collected in the blue channel.

Within the Image Viewer, the contrast and brightness can be adjusted and the individual color channels can be turned on and off by moving both range sliders in a channel to zero.

Keyboard Commands

In the High Resolution Image Viewer, use the keyboard commands to navigate through the image series to keep desired pan and zoom selections activated. Keyboard commands include:

You can also use the arrow keys to pan the current image.