The inaugural release of the Allen Brain Observatory aims to elucidate visual coding: How are visual stimuli represented by neural activity in the mouse visual cortex in both single cells and populations? To answer this question two-photon calcium imaging was recorded from several areas of the visual cortex in four distinct transgenic mouse lines during exposure of five rich visual stimuli.
Creating a Cortical Activity Map
To produce standardized protocols to be able to record from the same cortical area over the extent of the experiment required coordination and hand-off between several teams. A basic workflow is outlined below.