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Video recordings of the visual cortex were gathered during presentation of the various visual stimuli. Activity of the neurons in response to stimuli was detected as transient increases in cellular fluorescence on a millisecond time scale. The data processing of these raw movies involved motion correction and image segmentation to identify the sets of pixels representing distinct cells, and activity of these identified neurons was extracted as traces for quantification. Ultimately, the activity of each responsive cell may be correlated to the visual stimulus that was viewed by the mouse.

 

Motion-corrected 2-photon calcium fluorescence movies are too large to download conventionally, but are available upon request. Each experiment contains three movie files of approximately 60 GB each. We ask that anyone requesting movies provide one or more hard drives with sufficient capacity to store the files and a shipping number from your organization for return. These will be returned after file transfer is complete. Data is provided under the Allen Institute Terms of Use policy.

To request movie files, please contact us to indicate:

  • desired movies from specific experiments
  • point of contact name and email address
  • organization
  • area of research
  • proposed use of the data

Upon receipt of this message, arrangements will be made with the point of contact and will usually be complete within two weeks of the receipt of a storage drive.

Exploring the data

To learn more about the visual stimulus set as well as the data visualizations that were created to represent the cellular responses, see the visual stimulus pages:

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