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Being able to consistently locate a cortical region and even a specific cell requires that each system used to collect data maintains a consistent relationship between the mouse's eye and the visual stimulus being presented. To achieve consistent placement of the mouse across systems, and address the challenge of finding and returning to the same cells from day to day, the engineering team designed a custom headframe that is affixed to the mouse’s skull in a standardized location. The headframe consists of a plastic head plate, or well, and a stainless steal clamp-plate that allows repeatable positioning of the animal on each experimental setup. The clamp plate fits perfectly into the placement tool, which in turn is secured to the stereotaxic arm of the surgery rig. Before attaching the head frame, the surgeons use used a leveling protocol that is was anatomically referenced, so that the headframe is was consistently placed over the visual cortex. The cranial window is was made within the well to expose the part of the brain that we want was to imagebe imaged. This 3D image illustrates the final ensures an optimal position of the headframe that ensures we have most or all visual areas accessible within the windowto give visual access to the desirable cortical areas.