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A standardized clamping system on each experimental platform was made to match the head frame clamp plate and ensure reproducible placement of the head and eye position on all platforms. This cross platform registration is essential to put all the data into a common reference frame for downstream analysis.

Intrinsic Signal Imaging (ISI)

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Targeting functional areas of the visual cortex

Image Added Intrinsic signal imaging (ISI) measures the hemodynamic response of the cortex to visual stimulation across the entire field of view. This "retinotopic map" effectively represents the spatial relationship of the visual field to locations within each cortical area. Retinotopic mapping was used to delineate functionally defined visual area boundaries, and enabled targeting of the in vivo two-photon calcium imaging to functionally defined locations in primary and secondary visual cortical areas.