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A standardized clamping system on each experimental platform was made to match the head frame clamp plate and ensure reproducible placement of the head and eye position on all platforms. This cross platform registration standardization is essential to put register all the data into a common reference frame for downstream analysis.

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Visual Stimulus and Neuronal Response

Once a standard protocol and equipment were developed, activity of individual neurons to a visual stimulus in a targeted area of the visual cortex can be conducted.

Transgenic mouse lines

Neuronal activity was measured using For a