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Video recordings of the visual cortex were gathered during presentation of the various visual stimuli. Activity of the neurons in response to stimuli was detected as transient increases in cellular fluorescence on a millisecond time scale. The data processing of these raw movies involved motion correction and image segmentation to identify the sets of pixels representing distinct cells, and activity of these identified neurons was extracted as traces for quantification. Ultimately, the activity of each responsive cell may be correlated to the visual stimulus that was viewed by the mouse.

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