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Ivy Glioblastoma Studies

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Study

Description

Anatomic Structures ISH Survey

Primary screen of 8 tumors with probes for 343 genes enriched in glioblastoma.

Anatomic Structures RNA-Seq

Screen of 5 structures (Leading Edge, Infiltrating Tumor, Cellular Tumor, Microvascular Proliferation, and Pseudopalisading Cells Around Necrosis) identified by H&E staining. A total of 122 RNA samples were generated from 10 tumors.

Anatomic Structures ISH for Enriched Genes

Subsequent screen of 29 tumors with probes for 37 genes enriched in glioblastoma structures identified in Anatomic Structures RNA-Seq Study.

Cancer Stem Cells ISH Survey

Primary screen of 16 tumors with probes for 55 genes enriched in putative cancer stem cells, resulting in a 20 probe reference set, which was then used in an extensive screen of 42 tumors.

Cancer Stem Cells RNA-Seq

Screen of 35 clusters of putative cancer stem cells identified by ISH with a 17 reference probe subset (validated in the Cancer Stem Cells ISH Survey). A total of 148 RNA samples were generated from 34 tumors.

Cancer Stem Cells ISH for Enriched Genes

Subsequent screen of 37 tumors with probes for 76 genes enriched in clusters of putative cancer stem cells identified in the Cancer Stem Cells RNA-Seq Study.

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