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Representative retinas were sectioned in the vertical plane for characterization of morphology and co-localization with markers for well defined retinal ganglion cell (RGC) types. Four markers were used; VAChT (vesicular acetylcholine transporter) - a marker for cholinergic neuronsof inner plexiform layers, CART (cocaine-amphetamine related transcript) - a marker for granule ON/OFF direction selective retinal ganglion cells, OPN osteopontin - a marker of large/ dephosphorylated neurofilament-positive RGCs, and Brn3a - which labels ~80% of all RGCs and is a Pit/Oct/Unc (POU) domain transcription factor. Each experiment shows the GFP viral tracer infection in green, the marker in red and a DAPI stain in blue.

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